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Protocols and Custom Labware for OT-2 Opentrons Robot

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MLabS OT-2 protocols and designs.

These are most of the material I'm working on to use an OT-2 Opentrons Unit at my lab. Mostly, protocols I'm writing to try to automate some of the laboratory processes in the Microbiology Lab (www.arpbigidisba.com) at Balearic Institute of Sanitary Research (IdISBa - www.idisba.com) but also some custom designed labware and ideas to use with an OT-2 unit of Opentrons Robot (@Opentrons).

Please feel free to download, modify and or use any of these, but DO IT AT YOUR OWN RESPONSABILITY, protocols should have been tested properly, but there will be also Work In Progress protocols to kind of gather some help, feedback or advice.

Also, some labware I'm currently using is custom labware designed and 3D printed ad hoc to use with these protocols, you can find many of the files needed in the GitHub repository, please contact me at <gabriel.cabot(at)ssib.es> and I'll try to upload or reach to you.

Protocol Respository

  • Microdillution v.1.0 - Protocol to prepare 6x (96-well U-bottom plates with decreasing concentrations of (initially) antibiotics to perform an Antimicrobial Susceptibility Testing (AST) by Microdillution.

Custom Labware Repository

  • Reservoires (1-well, 8-well, 12-well, 96-well) for reagents.

Warning

Use these material under YOUR OWN responsability. I'll not be responsible for any damage caused at your OT-2 robot, labware, facility or person derived by the use of any of these material. THESE ARE Custom Labware and OT-2 Protocols, feel free to give any advice, feedback or ask anything.

ARPBIG_MLabS'24

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